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Roman Pioppino Mushroom Spawn Kit

Original price was: R1,399.Current price is: R995.

✔ Roman Pioppino Mushroom Spawn Kit (Cyclocybe aegerita)
✔ Includes 100 ml Living Inoculant (LI) in a reusable Culture Bottle
✔ Includes 1 kg Sterilized Sorghum Grain Spawn with SHIP & 0.2 µm PTFE filter
✔ Traditional Italian gourmet mushroom prized for its rich flavour and excellent texture

Delivery by:5 October

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Commercial 100cc Living Inoculant in Septum Bottle + Sterilized Sorghum Grain Spawn w/ SHIP

🌳 The Ancient Gourmet of the Poplar Tree

  • The Roman Pioppino Mushroom (Cyclocybe aegerita, formerly Agrocybe aegerita) is a classic Mediterranean gourmet species with a cultivation history dating back centuries. Traditionally grown on poplar logs throughout Italy, it is prized for its elegant appearance, firm texture and rich nutty flavour that develops beautifully during cooking.

    Producing dense clusters of chestnut-brown caps with long, slender stems, Pioppino has become a favourite of gourmet chefs for its ability to retain texture while adding a deep earthy aroma to stir-fries, pasta dishes and risottos.

    Each Spawn Kit contains everything required to establish your first grain spawn. Once fully colonized, the grain spawn can be expanded into your preferred hardwood substrate for productive gourmet harvests.


    🌱 Cultivation

    Roman Pioppino is a hardwood-loving species that performs exceptionally well on supplemented sawdust and Master’s Mix.

    Recommended substrates include:

    • Master’s Mix (Hardwood Sawdust & Soy Hulls)
    • Supplemented hardwood sawdust
    • Hardwood pellets
    • Hardwood logs
    • Poplar, oak and other deciduous hardwoods

    Pioppino colonizes steadily before producing attractive clusters of dark brown caps with slender stems. It benefits from high humidity, good Fresh Air Exchange (FAE) and indirect lighting during fruiting.

    Compared with oyster mushrooms, Pioppino develops more slowly but rewards growers with dense, long-lasting mushrooms of exceptional culinary quality.


    🇿🇦 Why South African Growers Love It

    • Traditional Italian gourmet mushroom
    • Rich nutty flavour with firm texture
    • Excellent shelf life after harvest
    • Attractive chestnut-brown mushroom clusters
    • Ideal for hardwood substrates
    • Premium restaurant-quality mushroom
    • A rewarding addition to any gourmet mushroom collection

    📦 What’s Included

    Each Roman Pioppino Spawn Kit contains:

  • 1 × 100 ml Living Inoculant (LI) containing Cyclocybe aegerita
    • Amber laboratory Culture Bottle with reusable 3 mm silicone septum
  • 1 × 1 kg Sterilized Sorghum Grain Spawn
    • Integrated Self-Healing Injection Port (SHIP)
    • 0.2 µm PTFE filter patch
  • 1 × Sterile 5 ml syringe
  • 1 × Sterile 16G needle

  • 🇮🇹 A Taste of the Mediterranean

    Few cultivated mushrooms carry such a rich culinary heritage as Pioppino. For generations it has been cultivated on poplar logs throughout Italy, where its distinctive flavour and elegant appearance earned it a place in traditional Mediterranean cuisine.

    Its firm texture allows it to hold its shape during cooking, while its rich earthy flavour complements everything from simple pasta dishes to slow-cooked stews and grilled vegetables.

    For growers seeking a premium gourmet mushroom with both history and exceptional eating quality, Roman Pioppino remains one of the finest hardwood species available.

Detailed instructions for using your Living Inoculant are provided in the “How to Use LI” tab.

How to Use LI

🧪 How to Use Your Living Inoculant (LI)

Your Spawn Kit has been designed to make mushroom cultivation as simple and contamination-resistant as possible. The included Living Inoculant (LI) contains an actively growing mushroom mono-culture, while the Sterilized Sorghum Grain Spawn provides the ideal medium for expanding that culture into vigorous grain spawn.


🧼 Step 1 – Sanitize

Before inoculation, wipe both the Culture Bottle septum and the SHIP (Self-Healing Injection Port) on the Spawn Bag with 70% rubbing alcohol.

Allow both surfaces to air dry before proceeding.


💉 Step 2 – Withdraw the Living Inoculant

Using the supplied sterile syringe and needle:

  • Insert the needle through the bottle septum.
  • Withdraw approximately 5 ml (5 cc) of Living Inoculant.
  • Remove any excess air from the syringe.

The Culture Bottle is reusable and can be accessed multiple times using sterile technique, via a 3mm thick silicone septum.


📦 Step 3 – Inoculate the Spawn Bag

Insert the needle through the SHIP on the Spawn Bag and slowly inject the 5 ml of Living Inoculant into the sterilized sorghum grain.

Remove the needle and gently massage the inoculation site if necessary.

The SHIP will reseal automatically after the needle is removed.


🌙 Step 4 – Incubate

Place the Spawn Bag in a clean, dark location at approximately 23–27°C.

Do not expose the bag to direct sunlight.

During incubation, the white mycelium will gradually spread throughout the grain.


🔄 Step 5 – Mix the Grain

Once approximately 30–40% of the grain has colonized with healthy white mycelium, gently break apart the colonized grain and mix it evenly throughout the bag.

This redistributes the growing mycelium and significantly accelerates complete colonization.


🍄 Step 6 – Use as Grain Spawn

Once the grain is fully colonized (completely white), it is ready to inoculate your preferred mushroom substrate.

Suitable substrates include pasteurized straw, hardwood sawdust, Master’s Mix, and other compatible materials depending on the mushroom species.

For best results, use the colonized grain spawn within 3 months.


💡 Tips for Success

  • Work as cleanly as possible during inoculation.
  • Always sanitize the bottle septum and SHIP before each use.
  • Do not remove or damage the filter patch.
  • Healthy mycelium should appear bright white and vigorous.
  • If contamination develops, dispose of the bag without opening it indoors.

♻️ Expanding Your Culture

One of the advantages of the Living Inoculant system is that the Culture Bottle contains enough inoculant to prepare multiple Spawn Bags.

When you’re ready to expand your production, simply order additional Sterilized Sorghum Grain Spawn and repeat the inoculation process using the remaining Living Inoculant.

How to use Living Inoculant (LI)

Weight 0.15 kg
Dimensions 20 × 8 × 13 cm
Culture Type

50cc Black PearlZ, 100cc liquid

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Pioppino Mushroom Agrocybe AegerhitaRoman Pioppino Mushroom Spawn Kit
Original price was: R1,399.Current price is: R995.

Delivery by:5 October

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