STS Cultivation Trials

Eco Bini™ BioaKt has been evaluated extensively during the development of the Bundu Tek cultivation system.

The following results represent actual production trials carried out using exact-hydration substrate preparation methods.

Trial Highlights

✔ Average 135.82% Biological Efficiency (BE) on White Oyster.

✔ Successful cultivation of Lion’s Mane on 100% South African Pine Pellets.

✔ Record White Oyster fruit bodies produced on engineered pine pellet media.

BioaKt Performance Data (White Oyster – Summer 2023):

Tests show the dramatic increase due to Alginates in new BioaKt – with 6% Ground Kelp.

Conventional Baseline: Treated Substrates (No BioaKt)

These results represent standard performance on various substrate mixes without the “Selective Doorkeeper” advantage. The average Biological Efficiency (BE) for these runs was 68.04%.

SP – Straw Pellets, CP – Chandy Pellets, CD-Coco Coir dust

2. Lion’s Mane on Pine: The “Impossible” Substrate

Traditional mycology suggests Lion’s Mane cannot thrive on Pine due to antifungal tannins and resins.

By using Bundu Teq and 6% BioaKt, we successfully neutralized these inhibitors, allowing for successful cultivation on 100% South African Pine Pellets using simple “Low-Tek” bucket methods.

3. Master’s Mix Equivalent: Breaking Records

We engineered a Pine Pellet mix to match the Nitrogen profile of the legendary “Master’s Mix” (1.1% N) with Bokashi Bam! Conola Meal. Using 3% BioaKt and Bundu Tek (strictly no sterilization), we obliterated our previous White Oyster record of 260g by almost 30%, producing a massive 330g single fruit body.

This proves that Selective Nutrition and Biological Occupancy can outperform the energy-intensive autoclave.

Pine Pellet Mushroom Substrates

BioaKt was specifically developed for engineered pine pellet media.

Unlike traditional lime pasteurisation, Bundu Tek uses exact hydration, where all of the process water remains within the substrate.

BioaKt performs several functions simultaneously:

• Alkaline pasteurisation
• pH buffering
• Nutritional supplementation
• Calcium enrichment
• Sulphur supplementation
• Natural kelp micronutrients

Recommended dosage

5% by dry substrate weight or 50 g (approximately 5 level tablespoons) per kilogram of dry pine pellets.

Other Substrates:

• HWFP or Straw Pellets – 1%
• Coco coir (CVG) – 3%

Coco Coir Conditioning

BioaKt may also be used to prepare coco coir before use.

Typical benefits include

• buffering pH
• calcium enrichment
• alkaline pasteurisation
• natural kelp micronutrients

Recommended dosage

3% or 30 g (approximately 3 tablespoons) per kilogram of dry coir.

1. Add the Bioakt to Coco Coir in bucket.
2. Add hydrating water.
3. Allow to condition for 24–36 hours.
4. Flush Coco Coir with 10L water per kilogram dry coco to remove the displace Na⁺ ions
5. Use

Experimental Applications

BioaKt continues to be evaluated for additional engineered media applications including

• True-Syn™ grainless spawn systems
• Specialty mushroom media
• Novel substrate engineering methods

Experimental applications are continually documented through STS Lab Notes.

Four Ingredients. Four Functions.

Self-Sanitising Nutrient Matrix

Unlike conventional nutritional supplements such as bran or soy hulls, BioaKt’s formulation undergoes a self-sanitising period after packaging.

This allows nutritional components to be safely incorporated into exact-hydration media without introducing untreated organic supplements.

Why Exact Hydration?

Traditional lime pasteurisation uses excess water, which is drained after treatment.

Bundu Tek instead hydrates processed substrates to their final working moisture content from the beginning.

This provides

• consistent hydration
• reduced handling
• no nutrient loss through draining
• a simpler and more repeatable preparation process.

What is Bundu Tek?

Bundu Tek is Eco Bini’s engineered substrate preparation system for gourmet mushroom cultivation.

Instead of relying on pressure sterilisers and contamination-prone supplementation, Bundu Tek combines carefully controlled hydration, alkaline conditioning and engineered media chemistry to produce consistent cultivation substrates using simple equipment.

Core principles include

• Exact hydration
• Engineered media conditioning
• Biological occupancy
• Resource-efficient cultivation
• Locally available substrate materials

BioaKt forms the foundation of this preparation system and was developed specifically for processed substrates such as pine pellets and coco coir.

For a detailed discussion of the Bundu Tek methodology, please refer to the accompanying STS Lab Notes.